- Title
- Chronic acrylamide exposure in male mice induces DNA damage to spermatozoa; potential for amelioration by resveratrol
- Creator
- Katen, Aimee L.; Stanger, Simone J.; Anderson, Amanda L.; Nixon, Brett; Roman, Shaun D.
- Relation
- Reproductive Toxicology Vol. 63, p. 1-12
- Publisher Link
- http://dx.doi.org/10.1016/j.reprotox.2016.05.004
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2016
- Description
- Humans are chronically exposed to acrylamide since carbohydrate rich foods contain the toxicant as aresult of cooking at high temperatures. While acrylamide is unreactive with DNA, it is readily oxidised toglycidamide, which adducts with DNA. This metabolism occurs via the enzyme, cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1). Acrylamide was administered to male CD1 mice for three or sixmonths at a dose of 0.18 mg/kg bodyweight/day. DNA damage was detected in germ cells and maturespermatozoa of exposed mice without compromising their overall fertility. The use of resveratrol, anantioxidant and known CYP2E1 inhibitor, was found to ameliorate the DNA damage in both germ cells andspermatozoa. However, extended resveratrol treatment (six months, 10.0 mg/kg bw/week) resulted inpremature activation of these cells. Thus the DNA damage found in spermatozoa after chronic acrylamideadministration can be alleviated but an alternative CYP2E1 inhibitor may be required.
- Subject
- acrylamide; glycidamide; resveratrol; DNA damage; CYP2E1; spermatozoa
- Identifier
- http://hdl.handle.net/1959.13/1336855
- Identifier
- uon:27718
- Identifier
- ISSN:0890-6238
- Language
- eng
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